RT Journal Article
SR Electronic
T1 Arabidopsis PAD4 lipase-like domain is a minimal functional unit in resistance to green peach aphid
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 769125
DO 10.1101/769125
A1 Joram A. Dongus
A1 Deepak D. Bhandari
A1 Monika Patel
A1 Lani Archer
A1 Lucas Dijkgraaf
A1 Laurent Deslandes
A1 Jyoti Shah
A1 Jane E. Parker
YR 2019
UL http://biorxiv.org/content/early/2019/09/14/769125.abstract
AB Plants have evolved mechanisms to attract beneficial microbes and insects while protecting themselves against pathogenic microbes and pests. In Arabidopsis, the immune regulator PAD4 functions with its cognate partner EDS1 to limit pathogen growth. PAD4, independently of EDS1, reduces infestation by Green Peach Aphid (GPA). How PAD4 regulates these defense outputs is unclear. By expressing the N-terminal PAD4-lipase-like domain (LLD) without its C-terminal ‘EDS1-PAD4’ (EP) domain, we interrogated PAD4 functions in plant defense. Here we show that transgenic expression of PAD4LLD in Arabidopsis is sufficient for limiting GPA infestation, but not for conferring basal and effector-triggered pathogen immunity. This suggests that the C-terminal PAD4-EP domain is necessary for EDS1-dependent immune functions. Moreover, PAD4LLD is not sufficient to interact with EDS1, indicating the PAD4-EP domain is required for heterodimerisation. These data provide molecular evidence that PAD4 has domain specific functions.