PT  - JOURNAL ARTICLE
AU  - Muhammad Adnan Shahzad
TI  - Mechanical unfold and transport of Green Florescent Protein through a nanopore
AID  - 10.1101/294819
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 294819
4099  - http://biorxiv.org/content/early/2018/04/04/294819.short
4100  - http://biorxiv.org/content/early/2018/04/04/294819.full
AB  - We report the unfold and trans-location of Green Fluorescent protein (GFP) mechanically by a constant force acting parallel along the axis of nanopore. A coarse-grained numerical model (Go-model) were implemented both for the protein and the nanopore. Detail description of each peptide unfold by the constant force is presented. Depending on the GFP topological structure, β-sheet barrel, the protein unfold and transport as a double loop conformation in the confinement geometry. The result is compared with maltose binding protein (MBP), having majority of alpha helix, which unfold and trans-locate as single profile conformation through nanopore. The result emphasis that protein with different topological structure unfold and trans-locate in different fashion depending on their native fold structure.