TY - JOUR T1 - Cytokinin perception in potato: New features of canonic players JF - bioRxiv DO - 10.1101/269266 SP - 269266 AU - Sergey N. Lomin AU - Yulia A. Myakushina AU - Oksana O. Kolachevskaya AU - Irina A. Getman AU - Dmitry V. Arkhipov AU - Ekaterina M. Savelieva AU - Dmitry I. Osolodkin AU - Georgy A. Romanov Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/04/269266.abstract N2 - Potato is the most economically important non-cereal food crop. Tuber formation in potato is regulated by phytohormones, cytokinins (CKs) in particular. The present work was aimed to study CK signal perception in potato. The sequenced potato genome of doubled monoploid Phureja was used for bioinformatic analysis and as a tool for identification of putative CK receptors from autotetraploid potato cv. Désirée. All basic elements of multistep phosphorelay (MSP) required for CK signal transduction were identified in Phureja genome, including three genes orthologous to three CK receptor genes (AHK 2-4) of Arabidopsis. As distinct from Phureja, autotetraploid potato contains at least two allelic isoforms of each receptor type. Putative receptor genes from Désirée plants were cloned, sequenced and expressed, and main characteristics of encoded proteins, firstly their consensus motifs, structure models, ligand-binding properties, and the ability to transmit CK signal, were determined. In all studied aspects the predicted sensor histidine kinases met the requirements for genuine CK receptors. Expression of potato CK receptors was found to be organ-specific and sensitive to growth conditions, particularly to sucrose content. Our results provide a solid basis for further in-depth study of CK signaling system and biotechnological improvement of potato.Abbreviationsaaamino acidBA6-benzyladenineCHASECyclases/Histidine kinases Associated SEnsoryCHKCHASE domain-containing histidine kinasesCKcytokininCRFcytokinin response factorcZcis-zeatinDIdimerization interfaceDZdihydrozeatinGFPgreen fluorescent proteinHKhistidine kinaseiPisopentenyladenineLacZgalactosidaseLDlong dayMSPmultistep phosphorelayRRresponse regulatorSNPsingle nucleotide polymorphismTDthidiazuronTMtransmembranetZtrans-zeatin. ER -