RT Journal Article
SR Electronic
T1 Molecular architecture of the mouse nervous system
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 294918
DO 10.1101/294918
A1 Amit Zeisel
A1 Hannah Hochgerner
A1 Peter Lönnerberg
A1 Anna Johnsson
A1 Fatima Memic
A1 Job van der Zwan
A1 Martin Häring
A1 Emelie Braun
A1 Lars Borm
A1 Gioele La Manno
A1 Simone Codeluppi
A1 Alessandro Furlan
A1 Nathan Skene
A1 Kenneth D. Harris
A1 Jens Hjerling Leffler
A1 Ernest Arenas
A1 Patrik Ernfors
A1 Ulrika Marklund
A1 Sten Linnarsson
YR 2018
UL http://biorxiv.org/content/early/2018/04/06/294918.abstract
AB The mammalian nervous system executes complex behaviors controlled by specialised, precisely positioned and interacting cell types. Here, we used RNA sequencing of half a million single cells to create a detailed census of cell types in the mouse nervous system. We mapped cell types spatially and derived a hierarchical, data-driven taxonomy. Neurons were the most diverse, and were grouped by developmental anatomical units, and by the expression of neurotransmitters and neuropeptides. Neuronal diversity was driven by genes encoding cell identity, synaptic connectivity, neurotransmission and membrane conductance. We discovered several distinct, regionally restricted, astrocytes types, which obeyed developmental boundaries and correlated with the spatial distribution of key glutamate and glycine neurotransmitters. In contrast, oligodendrocytes showed a loss of regional identity, followed by a secondary diversification. The resource presented here lays a solid foundation for understanding the molecular architecture of the mammalian nervous system, and enables genetic manipulation of specific cell types.