TY - JOUR T1 - Core architecture of a bacterial type II secretion system JF - bioRxiv DO - 10.1101/397794 SP - 397794 AU - Anastasia A Chernyatina AU - Harry H Low Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/09/17/397794.abstract N2 - Bacterial type II secretion systems (T2SS) translocate virulence factors, toxins and enzymes across the cell outer membrane (OM). An assembled T2SS has not yet been isolated in vitro. Here we use a fusion of negative stain and cryo-electron microscopy (EM) to reveal the core architecture of an assembled T2SS from the pathogen Klebsiella pneumoniae. We show that 7 proteins form a ∼2.5 MDa complex that spans the cell envelope. The outer membrane complex (OMC) includes the secretin PulD with all domains modelled and the pilotin PulS. The inner membrane assembly platform (AP) components PulC, PulE, PulL, PulM and PulN have a relative stoichiometric ratio of 2:1:1:1:1, respectively. The PulE ATPase, PulL and PulM combine to form a flexible hexameric hub. Symmetry mismatch between the OMC and AP is overcome by PulC linkers spanning the periplasm with PulC HR domains binding independently at the secretin base. Our results show the T2SS to have a highly dynamic modular architecture with implication for pseudo-pilus assembly and substrate loading. ER -