RT Journal Article
SR Electronic
T1 CrispRVariants: precisely charting the mutation spectrum in genome engineering experiments
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 034140
DO 10.1101/034140
A1 Helen Lindsay
A1 Alexa Burger
A1 Anastasia Felker
A1 Christopher Hess
A1 Jonas Zaugg
A1 Elena Chiavacci
A1 Carolin Anders
A1 Martin Jinek
A1 Christian Mosimann
A1 Mark D. Robinson
YR 2015
UL http://biorxiv.org/content/early/2015/12/10/034140.abstract
AB CRISPR-Cas9 and related technologies efficiently alter genomic DNA at targeted positions and have far-reaching implications for functional screening and therapeutic gene editing. Understanding and unlocking this potential requires accurate evaluation of editing efficiency. We show that methodological decisions for analyzing sequencing data can significantly affect mutagenesis efficiency estimates and we provide a comprehensive R-based toolkit, CrispRVariants, that resolves and localizes individual mutant alleles with respect to the endonuclease cut site. CrispRVariants-enabled analyses of newly generated and existing genome editing datasets underscore how careful consideration of the full variant spectrum gives insight toward effective guide and amplicon design as well as the mutagenic process.