RT Journal Article
SR Electronic
T1 Imaging plant germline differentiation within Arabidopsis flower by light sheet microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 774299
DO 10.1101/774299
A1 Valuchova, Sona
A1 Mikulkova, Pavlina
A1 Pecinkova, Jana
A1 Klimova, Jana
A1 Krumnikl, Michal
A1 Bainar, Petr
A1 Heckmann, Stefan
A1 Tomancak, Pavel
A1 Riha, Karel
YR 2019
UL http://biorxiv.org/content/early/2019/09/19/774299.abstract
AB In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.