RT Journal Article
SR Electronic
T1 Nuclear actin cable with multiple branches during yeast meiosis
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 778100
DO 10.1101/778100
A1 Tomoko Takagi
A1 Masako Osumi
A1 Akira Shinohara
YR 2019
UL http://biorxiv.org/content/early/2019/09/21/778100.abstract
AB Actin polymerizes to form filaments/cables for motility, transport, and structural framework in a cell. Recent studies show that actin polymers are present not only in cytoplasm, but also in nuclei of vertebrate cells, and their formation is induced in response to stress. Here, by electron microscopic observation with rapid freezing and high-pressure freezing, we found a unique polymerized form of actin inside of nuclei of budding yeast cells undergoing meiosis. The nuclear actin cable during meiosis consists of several actin filaments with a rectangular lattice arrangement and is associated with multiple branched cables/filaments showing “feather-like” appearance. The cable is immuno-labeled with anti-actin antibody and sensitive to an actin-depolymerizing drug. Like cytoplasmic actin cables, nuclear actin cables are rarely seen in pre-meiotic cells and spores, and are induced during meiotic prophase-I. We speculate that nuclear actin cables play a role in nuclear events during meiotic prophase I.