RT Journal Article
SR Electronic
T1 Identification of PTBP1 responsible for caspase dependent YRNA cleavage
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 298851
DO 10.1101/298851
A1 Jun Ogata
A1 Yuki Sugiura
A1 Akinori Kanai
A1 Masafumi Tanaka
A1 Hirotaka Matsui
A1 Masato Ohtsuka
A1 Toshiya Inaba
A1 Motoyuki Otsuka
A1 Ai Kotani
YR 2018
UL http://biorxiv.org/content/early/2018/04/10/298851.abstract
AB Some RNAs such as 28S rRNA, U1 snRNA, and Y RNAs are known to be cleaved during apoptosis. As the underlying mechanism is yet unclear, the functions and biological significance of RNA degradation in apoptosis remain elusive. We previously identified novel, functional small RNAs named AGO-taxis small RNA (ASR) that are specifically bound to AGO1. Here, we investigated ASR biogenesis, which appears to be non-canonical. Y RNAs, non-coding RNAs degraded during apoptosis, were identified as the precursors of several ASRs. Cell-free analysis combined with fractionation methods revealed that the apoptosis-specific biogenesis of ASRs or Y RNA degradation was induced by PTBP1—an endoribonuclease inhibitor of Y RNAs. PTBP1, a splicing factor, was truncated by caspase 3, which subsequently activated endoribonuclease to induce biogenesis of ASRs and Y RNA cleavage.