PT  - JOURNAL ARTICLE
AU  - Pandey, Pratima
AU  - Singh, Anuradha
AU  - Kaur, Harjot
AU  - Ghosh-Roy, Anindya
AU  - Babu, Kavita
TI  - Loss of <em>dop-2</em> causes increased dopamine release and locomotory defects in the presence of ethanol
AID  - 10.1101/779405
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 779405
4099  - http://biorxiv.org/content/early/2019/09/23/779405.short
4100  - http://biorxiv.org/content/early/2019/09/23/779405.full
AB  - Ethanol is a widely used drug, excessive consumption of which could lead to medical conditions with diverse symptoms. Ethanol abuse causes disinhibition of memory, attention, speech and locomotion across species. Dopamine signaling plays an essential role in ethanol dependent behaviors in animals ranging from C. elegans to humans. We devised an ethanol dependent assay in which mutants in the dopamine autoreceptor, dop-2, displayed a unique sedative locomotory behavior causing the animals to move in circles while dragging the posterior half of their body. We identify the posterior dopaminergic sensory neuron as being essential to modulate this behavior. We further demonstrate that in dop-2 mutants, ethanol exposure increases dopamine secretion and results in enhanced function of the DVA interneuron. DVA releases the neuropeptide NLP-12 and leads to the excitation of cholinergic motor neurons that affect movement. Thus, DOP-2 modulates dopamine levels at the synapse and regulates alcohol induced movement through NLP-12.