RT Journal Article
SR Electronic
T1 Integrated fusible alloy microelectrodes based microfluidic impedance cytometry for cell-in-droplet quantification
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 278069
DO 10.1101/278069
A1 Jatin Panwar
A1 Rahul Roy
YR 2018
UL http://biorxiv.org/content/early/2018/04/13/278069.abstract
AB Microfluidic impedance cytometry (MIC) provides a non-optical and label-free method for single cell analysis. However, the cleanroom intensive infrastructure required for MIC electrode fabrication limits its implementation. As an alternate approach that enables rapid prototyping, we fabricated Field’s metal (FM) ‘in-contact’ coplanar (icFM) microelectrodes in multilayer elastomer devices with a single photolithography step and characterised them for microfluidic impedance cytometry. Our icFM microelectrodes matched performance of the conventional platinum electrodes in the detection of single human erythrocytes and water-in-oil droplets in a feedback-controlled suction-flow MIC setup. Finally, to facilitate droplet based single cell analysis, we demonstrate detection and quantification of single erythrocytes entrapped in water-in-oil droplets.