RT Journal Article
SR Electronic
T1 Custom built scanner and simple image processing pipeline enables low-cost, high-throughput phenotyping of maize ears
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 780650
DO 10.1101/780650
A1 Cedar Warman
A1 John E Fowler
YR 2019
UL http://biorxiv.org/content/early/2019/09/27/780650.abstract
AB High-throughput phenotyping systems are becoming increasingly powerful, dramatically changing our ability to document, measure, and detect phenomena. Unfortunately, taking advantage of these trends can be difficult for scientists with few resources, particularly when studying nonstandard biological systems. Here, we describe a powerful, cost-effective combination of a custom-built imaging platform and open-source image processing pipeline. Our maize ear scanner was built with off-the-shelf parts for <$80. When combined with a cellphone or digital camera, videos of rotating maize ears were captured and digitally flattened into projections covering the entire surface of the ear. Segregating GFP and anthocyanin seed markers were clearly distinguishable in ear projections, allowing manual annotation using ImageJ. Using this method, statistically powerful transmission data can be collected for hundreds of maize ears, accelerating the phenotyping process.