PT  - JOURNAL ARTICLE
AU  - Pengju Zhang
AU  - Jun Wang
AU  - Hongyan Lang
AU  - Weixia Wang
AU  - Xiaohui Liu
AU  - Haiyan Liu
AU  - Chengcheng Tan
AU  - Xintao Li
AU  - Yumin Zhao
AU  - Xinghong Wu
TI  - MicroRNA-205 affects mouse granulosa cell apoptosis and estradiol synthesis by targeting CREB1
AID  - 10.1101/301523
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 301523
4099  - http://biorxiv.org/content/early/2018/04/14/301523.short
4100  - http://biorxiv.org/content/early/2018/04/14/301523.full
AB  - MicroRNAs-205 (miR-205), were reportedly to be involved in various physiological and pathological processes, but its biological function in follicular atresia remain unknown. In this study, we investigated the expression of miR-205 in mouse granulosa cells (mGCs), and explored its functions in primary mGCs using a serial of in vitro experiments. The result of qRT-PCR demonstrated that miR-205 expression was significantly increased in early atretic follicles (EAF), and progressively atretic follicles (PAF) compared to healthy follicles (HF). Our results also revealed that overexpression of miR-205 in mGCs significantly promoted apoptosis, caspas-3/9 activities, and inhibited estrogen E2 release, and cytochrome P450 family 19 subfamily A polypeptide 1 (CYP19A1, a key gene in E2 production) expression. Bioinformatics and luciferase reporter assays revealed that the gene of cyclic AMP response element (CRE)-binding protein 1 (CREB1) was a potential target of miR-205. qRT-PCR and western blot assays revealed that overexpression of miR-205 inhibited the expression of CREB1 in mGCs. Importantly, CREB1 upregulation partially rescued the effects of miR-205 on apoptosis, caspase-3/9 activities, E2 production and CYP19A1 expression in mGCs. Our results indicate that miR-205 may play an important role in ovarian follicular development and provide new insights into follicular atresia.