TY - JOUR T1 - Actin filaments regulate microtubule growth at the centrosome JF - bioRxiv DO - 10.1101/302190 SP - 302190 AU - Daisuke Inoue AU - Dorian Obino AU - Francesca Farina AU - Jérémie Gaillard AU - Christophe Guerin AU - Laurent Blanchoin AU - Ana-Maria Lennon-Duménil AU - Manuel Théry Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/16/302190.abstract N2 - The centrosome is the main microtubule-organizing centre. It also organizes a local network of actin filaments. However, the precise function of the actin network at the centrosome is not well understood. Here we show that increasing densities of actin filaments at the centrosome of lymphocytes were correlated with reduced amounts of microtubules. Furthermore, lymphocyte activation resulted in centrosomal-actin disassembly and an increase in microtubule number. To further investigate the direct crosstalk between actin and microtubules at the centrosome, we performed in vitro reconstitution assays based on (i) purified centrosomes and (ii) on the co-micropatterning of microtubule seeds and actin filaments. The two assays demonstrated that actin filaments perturb microtubule growth by steric hindrance. Finally, we showed that cell adhesion and spreading leads to lower densities of centrosomal actin thus resulting in higher microtubule growth. Hence we propose a novel mechanism by which the number of centrosomal microtubules is regulated by cell adhesion and actin-network architecture. ER -