TY - JOUR T1 - Festa: <em>F</em>Lexible <em>E</em>Xon-Based <em>S</em>Plicing And <em>T</em>Ranscription <em>A</em>Nnotation JF - bioRxiv DO - 10.1101/300947 SP - 300947 AU - Rago Alfredo AU - Cobourne John K. Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/17/300947.abstract N2 - We introduce FESTA, an R based algorithm that allows detection of alternative splicing based on experiment-specific exon expression data. FESTA disentangles alternative splicing signal from whole-gene transcription, facilitating the discovery and characterization of novel regulatory events even in the absence of transcript annotations or paired-end data. We also include customization options to increase its applicability on different platforms and experimental designs as well as a tool for the conversion from transcript expression to inclusion ratios. ER -