PT  - JOURNAL ARTICLE
AU  - Kae Koganebuchi
AU  - Takashi Gakuhari
AU  - Hirohiko Takeshima
AU  - Kimitoshi Sato
AU  - Kiyotaka Fujii
AU  - Toshihiro Kumabe
AU  - Satoshi Kasagi
AU  - Takehiro Sato
AU  - Atsushi Tajima
AU  - Hiroki Shibata
AU  - Motoyuki Ogawa
AU  - Hiroki Oota
TI  - A new targeted capture method using bacterial artificial chromosome (BAC) libraries as baits for sequencing relatively large genes
AID  - 10.1101/302760
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 302760
4099  - http://biorxiv.org/content/early/2018/04/17/302760.short
4100  - http://biorxiv.org/content/early/2018/04/17/302760.full
AB  - To analyze a specific genome region using next-generation sequencing technologies, the enrichment of DNA libraries with targeted capture methods has been standardized. For enrichment of mitochondrial genome, a previous study developed an original targeted capture method that use baits constructed from long-range polymerase chain reaction (PCR) amplicons, common laboratory reagents, and equipment. In this study, a new targeted capture method is presented, that of bacterial artificial chromosome (BAC) double capture (BDC), modifying the previous method, but using BAC libraries as baits for sequencing a relatively large gene. We applied the BDC approach for the 214 kb autosomal region, ring finger protein 213, which is the susceptibility gene of moyamoya disease (MMD). To evaluate the reliability of BDC, cost and data quality were compared with those of a commercial kit. While the ratio of duplicate reads was higher, the cost was less than that of the commercial kit. The data quality was sufficiently the same as that of the kit. Thus, BDC can be an easy, low-cost, and useful method for analyzing individual genome region with substantial length.