RT Journal Article
SR Electronic
T1 The roles of <em>hox 13</em> genes in newt limb development and regeneration
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 789180
DO 10.1101/789180
A1 Takashi Takeuchi
A1 Fumina Minamitani
A1 Kazuki Koriyama
A1 Yukio Satoh
A1 Ken-ichi Suzuki
A1 Shuji Shigenobu
A1 Takeshi Inoue
A1 Kiyokazu Agata
A1 Toshinori Hayashi
YR 2019
UL http://biorxiv.org/content/early/2019/10/01/789180.abstract
AB Posterior Hox genes play crucial roles in limb development and specify regions in the proximal-distal (PD) axis of limbs. However, there is no direct genetic evidence that Hox genes are essential for limb regeneration. Moreover, if essential, it is totally unknown which Hox genes have the same or distinct functions between development and regeneration. Here, we mutated hox13 using an efficient CRISPR/Cas9 system in newts (Pleurodeles waltl), which have strong regenerative capacities in various tissues. Triple or double mutants of hox13 paralogs lost their digit and metacarpal/metatarsal bones. Limb regeneration progressed but regenerates lacked the same autopod region. These results showed that hox13 paralogs have the same functions in limb development and regeneration.