PT  - JOURNAL ARTICLE
AU  - Velasco, Mary Grace M.
AU  - Zhang, Mengyang
AU  - Antonello, Jacopo
AU  - Yuan, Peng
AU  - Allgeyer, Edward S.
AU  - May, Dennis
AU  - M’Saad, Ons
AU  - Kidd, Phylicia
AU  - Barentine, Andrew E. S.
AU  - Greco, Valentina
AU  - Grutzendler, Jaime
AU  - Booth, Martin J.
AU  - Bewersdorf, Joerg
TI  - 3D super-resolution deep-tissue imaging in living mice
AID  - 10.1101/790212
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 790212
4099  - http://biorxiv.org/content/early/2019/10/02/790212.short
4100  - http://biorxiv.org/content/early/2019/10/02/790212.full
AB  - Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of 2-photon excitation, adaptive optics, far-red emitting organic dyes, and a long-working distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.