RT Journal Article
SR Electronic
T1 3D super-resolution deep-tissue imaging in living mice
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 790212
DO 10.1101/790212
A1 Velasco, Mary Grace M.
A1 Zhang, Mengyang
A1 Antonello, Jacopo
A1 Yuan, Peng
A1 Allgeyer, Edward S.
A1 May, Dennis
A1 M’Saad, Ons
A1 Kidd, Phylicia
A1 Barentine, Andrew E. S.
A1 Greco, Valentina
A1 Grutzendler, Jaime
A1 Booth, Martin J.
A1 Bewersdorf, Joerg
YR 2019
UL http://biorxiv.org/content/early/2019/10/02/790212.abstract
AB Stimulated emission depletion (STED) microscopy enables the three-dimensional (3D) visualization of dynamic nanoscale structures in living cells, offering unique insights into their organization. However, 3D-STED imaging deep inside biological tissue is obstructed by optical aberrations and light scattering. We present a STED system that overcomes these challenges. Through the combination of 2-photon excitation, adaptive optics, far-red emitting organic dyes, and a long-working distance water-immersion objective lens, our system achieves aberration-corrected 3D super-resolution imaging, which we demonstrate 164 µm deep in fixed mouse brain tissue and 76 µm deep in the brain of a living mouse.