RT Journal Article
SR Electronic
T1 Single-cell analysis of human retina identifies evolutionarily conserved and species-specific mechanisms controlling development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 779694
DO 10.1101/779694
A1 Yufeng Lu
A1 Fion Shiau
A1 Wenyang Yi
A1 Suying Lu
A1 Qian Wu
A1 Joel D. Pearson
A1 Alyssa Kallman
A1 Suijuan Zhong
A1 Thanh Hoang
A1 Zhentao Zuo
A1 Fangqi Zhao
A1 Mei Zhang
A1 Nicole Tsai
A1 Yan Zhuo
A1 Sheng He
A1 Jun Zhang
A1 Genevieve L. Stein-O’Brien
A1 Thomas D. Sherman
A1 Xin Duan
A1 Elana J. Fertig
A1 Loyal A. Goff
A1 Donald J. Zack
A1 James T. Handa
A1 Tian Xue
A1 Rod Bremner
A1 Seth Blackshaw
A1 Xiaoqun Wang
A1 Brian S. Clark
YR 2019
UL http://biorxiv.org/content/early/2019/10/02/779694.abstract
AB The development of single-cell RNA-Sequencing (scRNA-Seq) has allowed high resolution analysis of cell type diversity and transcriptional networks controlling cell fate specification. To identify the transcriptional networks governing human retinal development, we performed scRNA-Seq over retinal organoid and in vivo retinal development, across 20 timepoints. Using both pseudotemporal and cross-species analyses, we examined the conservation of gene expression across retinal progenitor maturation and specification of all seven major retinal cell types. Furthermore, we examined gene expression differences between developing macula and periphery and between two distinct populations of horizontal cells. We also identify both shared and species-specific patterns of gene expression during human and mouse retinal development. Finally, we identify an unexpected role for ATOH7 expression in regulation of photoreceptor specification during late retinogenesis. These results provide a roadmap to future studies of human retinal development, and may help guide the design of cell-based therapies for treating retinal dystrophies.