RT Journal Article
SR Electronic
T1 BMP signaling regulates Id1 mediated neural stem cell quiescence in the adult zebrafish brain via a phylogenetically conserved enhancer module
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 787804
DO 10.1101/787804
A1 Gaoqun Zhang
A1 Marco Ferg
A1 Luisa Lübke
A1 Tanja Beil
A1 Victor Gourain
A1 Nicolas Diotel
A1 Uwe Strähle
A1 Sepand Rastegar
YR 2019
UL http://biorxiv.org/content/early/2019/10/02/787804.abstract
AB In the telencephalon of adult zebrafish, the inhibitor of DNA binding 1 (id1) gene is expressed in radial glial cells (RGCs), behaving as neural stem cells (NSCs), during constitutive and regenerative neurogenesis. Id1 controls the balance between resting and proliferating states of RGCs by promoting quiescence. Here, we identified a phylogenetically conserved cis-regulatory module (CRM) mediating the specific expression of id1 in RGCs. Systematic deletion mapping and mutation of conserved transcription factor binding sites in stable transgenic zebrafish lines reveal that this CRM operates via conserved smad1/5 and 4 binding motifs (SBMs) under both homeostatic and regenerative conditions. Transcriptome analysis of injured and uninjured telencephala as well as pharmacological inhibition experiments identify a crucial role of bone morphogenetic protein (BMP) signaling for the function of the CRM. Our data highlight that BMP signals control id1 expression and thus NSC proliferation during constitutive and induced neurogenesis.