TY - JOUR T1 - Enhancing autophagy by redox regulation extends lifespan in <em>Drosophila</em> JF - bioRxiv DO - 10.1101/790378 SP - 790378 AU - Helena M. Cochemé AU - Ivana Bjedov AU - Sebastian Grönke AU - Katja E. Menger AU - Andrew M. James AU - Jorge Ivan Castillo Quan AU - Andrea Foley AU - Claudia Lennicke AU - Marcela Buricova AU - Jennifer Adcott AU - Filipe Cabreiro AU - Michael P. Murphy AU - Linda Partridge Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/10/02/790378.abstract N2 - Redox signalling is an important modulator of diverse biological pathways and processes, and operates through specific post-translational modification of redox-sensitive thiols on cysteine residues 1–4. Critically, redox signalling is distinct from irreversible oxidative damage and functions as a reversible ‘redox switch’ to regulate target proteins. H2O2 acts as the major effector of redox signalling, both directly and through intracellular thiol redox relays 5,6. Dysregulation of redox homeostasis has long been implicated in the pathophysiology of many age-related diseases, as well as in the ageing process itself, however the underlying mechanisms remain largely unclear 7,8. To study redox signalling by H2O2 in vivo and explore its involvement in metabolic health and longevity, we used the fruit fly Drosophila as a model organism, with its tractable lifespan and strong evolutionary conservation with mammals 9. Here we report that inducing an endogenous redox-shift, by manipulating levels of the H2O2-degrading enzyme catalase, improves health and robustly extends lifespan in flies, independently of oxidative stress resistance and dietary restriction. We find that the catalase redox-shifted flies are acutely sensitive to starvation stress, which relies on autophagy as a vital survival mechanism. Importantly, we show that autophagy is essential for the lifespan extension of the catalase flies. Furthermore, using redox-inactive knock-in mutants of Atg4a, a major effector of autophagy, we show that the lifespan extension in response to catalase requires a key redox-regulatory cysteine residue, Cys102 in Atg4a. These findings demonstrate that redox regulation of autophagy can extend lifespan, confirming the importance of redox signalling in ageing and as a potential pro-longevity target. ER -