RT Journal Article
SR Electronic
T1 Single cell map of the human ovarian cortex
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 791343
DO 10.1101/791343
A1 Magdalena Wagner
A1 Masahito Yoshihara
A1 Iyadh Douagi
A1 Anastasios Damdimopoulos
A1 Sarita Panula
A1 Sophie Petropoulos
A1 Haojiang Lu
A1 Karin Pettersson
A1 Kerstin Palm
A1 Shintaro Katayama
A1 Outi Hovatta
A1 Juha Kere
A1 Fredrik Lanner
A1 Pauliina Damdimopoulou
YR 2019
UL http://biorxiv.org/content/early/2019/10/02/791343.abstract
AB The human ovary orchestrates sex hormone production and undergoes monthly structural changes to release mature oocytes. The outer lining of the ovary (cortex) has a key role in defining fertility in women as it harbors the ovarian reserve. It has been postulated that putative oogonial stem cells exist in the ovarian cortex and that these can be captured by DDX4 antibody isolation. We analysed on a single cell level the transcriptome and cell surface antigen profiles of over 24,000 cells from high quality ovarian cortex samples from 21 patients. Our single cell mapping reveals transcriptional profiles of six main cell types; oocytes, granulosa cells, immune cells, endothelial cells, perivascular cells, and stromal cells. Cells captured by DDX4 antibody are perivascular cells, not oogonial stem cells. Our data does not support the existence of germline stem cells in adult human ovaries thereby reinforcing the dogma of a limited ovarian reserve.