RT Journal Article
SR Electronic
T1 A multicellular rosette-mediated collective dendrite extension
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 303503
DO 10.1101/303503
A1 Li Fan
A1 Ismar Kovacevic
A1 Maxwell G. Heiman
A1 Zhirong Bao
YR 2018
UL http://biorxiv.org/content/early/2018/04/18/303503.abstract
AB Coordination of neurite morphogenesis with surrounding tissues is crucial to the establishment of neural circuits, but the underlying cellular and molecular mechanisms remain poorly understood. We show that neurons in a C. elegans sensory organ, called the amphid, undergo a collective dendrite extension to initiate formation of the sensory nerve. The amphid neurons first assemble into a multicellular rosette. The vertex of the rosette, which becomes the dendrite tips, is attached to the anteriorly migrating epidermis and carried to the sensory depression, extruding the dendrites away from the neuronal cell bodies. Multiple adhesion molecules including DYF-7, SAX-7, HMR-1 and DLG-1 function redundantly in rosette-to-epidermis attachment. PAR-6 is localized to the rosette vertex and dendrite tips, and promotes DYF-7 localization and dendrite extension. Our results suggest a collective mechanism of neurite extension that is distinct from the classical pioneer-follower model and highlight the role of mechanical cues from surrounding tissues in shaping neurites.