PT  - JOURNAL ARTICLE
AU  - Monika Mohenska
AU  - Nathalia M. Tan
AU  - Alex Tokolyi
AU  - Milena B. Furtado
AU  - Mauro W. Costa
AU  - Andrew J. Perry
AU  - Jessica Hatwell-Humble
AU  - Karel van Duijvenboden
AU  - Hieu T. Nim
AU  - Susan K. Nilsson
AU  - David R. Powell
AU  - Nadia A. Rosenthal
AU  - Fernando J. Rossello
AU  - Mirana Ramialison
AU  - Jose M. Polo
TI  - 3D-Cardiomics: A spatial transcriptional atlas of the mammalian heart
AID  - 10.1101/792002
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 792002
4099  - http://biorxiv.org/content/early/2019/10/03/792002.short
4100  - http://biorxiv.org/content/early/2019/10/03/792002.full
AB  - Understanding spatial gene expression and regulation is key to uncovering developmental and physiological processes, during homeostasis and disease. Numerous techniques exist to gain gene expression and regulation information, but very few utilise intuitive true-to-life three-dimensional representations to analyze and visualize results. Here we combined spatial transcriptomics with 3D modelling to represent and interrogate, transcriptome-wide, three-dimensional gene expression and location in the mouse adult heart. Our study has unveiled specific subsets of genes that display complex spatial expression in organ sub-compartments. Also, we created a web-based user interface for spatial transcriptome analysis and visualization. The application may be accessed from http://3d-cardiomics.erc.monash.edu/.