TY - JOUR T1 - Characterisation and functional predictions of canine long non-coding RNAs JF - bioRxiv DO - 10.1101/303966 SP - 303966 AU - Céline Le Béguec AU - Valentin Wucher AU - Lætitia Lagoutte AU - Edouard Cadieu AU - Nadine Botherel AU - Benoît Hédan AU - Clotilde De Brito AU - Guillory Anne-Sophie AU - Catherine André AU - Thomas Derrien AU - Christophe Hitte Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/20/303966.abstract N2 - Long non-coding RNAs (lncRNAs) are a family of heterogeneous RNAs that play major roles in multiple biological processes. We recently identified an extended repertoire of more than 10,000 lncRNAs of the domestic dog however, predicting their biological functionality remains challenging. In this study, we have characterised the expression profiles of 10,444 canine lncRNAs in 26 distinct tissue types, representing various anatomical systems. We showed that lncRNA expressions are mainly clustered by tissue type and we highlighted that 44% of canine lncRNAs are expressed in a tissue-specific manner. We further demonstrated that tissue-specificity correlates with specific families of canine transposable elements. In addition, we identified more than 900 conserved dog-human lncRNAs for which we show their overall reproducible expression patterns between dog and humans through comparative transcriptomics. Finally, co-expression analyses of lncRNA and neighbouring protein-coding genes identified more than 3,400 canine lncRNAs, suggesting that functional roles of these lncRNAs act as regulatory elements. Altogether, this genomic and transcriptomic integrative study of lncRNAs constitutes a major resource to investigate genotype to phenotype relationships and biomedical research in the dog species. ER -