PT  - JOURNAL ARTICLE
AU  - Kristyn A. Robinson
AU  - Kenzie E. Pereira
AU  - Molly C. Bletz
AU  - Edward Davis Carter
AU  - Matthew J. Gray
AU  - Jonah Piovia-Scott
AU  - John M. Romansic
AU  - Douglas C. Woodhams
AU  - Lillian Fritz-Laylin
TI  - Isolation and maintenance of <em>Batrachochytrium salamandrivorans</em> cultures
AID  - 10.1101/796920
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 796920
4099  - http://biorxiv.org/content/early/2019/10/08/796920.short
4100  - http://biorxiv.org/content/early/2019/10/08/796920.full
AB  - Discovered in 2013, Batrachochytrium salamandrivorans (Bsal) is an emerging amphibian pathogen that causes ulcerative skin lesions and multifocal erosion. A closely related pathogen, Batrachochytrium dendrobatidis (Bd), has devastated amphibian populations worldwide, suggesting that Bsal poses a significant threat to global salamander biodiversity. To expedite research into this emerging threat, we seek to standardize protocols across the field so that results of laboratory studies are reproducible and comparable. We have collated data and experience from multiple labs to standardize culturing practices of Bsal. Here we outline common culture practices including a media for optimal Bsal growth, standard culture protocols, and a method for isolating Bsal from infected tissue.