RT Journal Article
SR Electronic
T1 Examining go-or-grow using fluorescent cell-cycle indicators and cell cycle-inhibiting drugs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 797142
DO 10.1101/797142
A1 Sean T. Vittadello
A1 Scott W. McCue
A1 Gency Gunasingh
A1 Nikolas K. Haass
A1 Matthew J. Simpson
YR 2019
UL http://biorxiv.org/content/early/2019/10/08/797142.abstract
AB The go-or-grow hypothesis states that adherent cells undergo reversible phenotype switching between migratory and proliferative states, with cells in the migratory state being more motile than cells in the proliferative state. Here, we examine go-or-grow using melanoma cells with fluorescent cell-cycle indicators and cell cycle-inhibiting drugs. We analyse the experimental data using single-cell tracking to calculate mean diffusivities, and compare motility between cells in different cell-cycle phases and in cell-cycle arrest. Unequivocally, our analysis does not support the go- or-grow hypothesis. We present clear evidence that cell motility is independent of the cell-cycle phase, and non-proliferative arrested cells have the same motility as cycling cells.