RT Journal Article
SR Electronic
T1 Wide-field time-gated SPAD imager for phasor-based FLIM applications
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 687277
DO 10.1101/687277
A1 Arin Ulku
A1 Andrei Ardelean
A1 Michel Antolovic
A1 Shimon Weiss
A1 Edoardo Charbon
A1 Claudio Bruschini
A1 Xavier Michalet
YR 2019
UL http://biorxiv.org/content/early/2019/10/09/687277.abstract
AB We describe the performance of a new wide area time-gated single-photon avalanche diode (SPAD) array for phasor-FLIM, exploring the effect of gate length, gate number and signal intensity on the measured lifetime accuracy and precision. We conclude that the detector functions essentially as an ideal shot noise limited sensor and is capable of video rate FLIM measurement. The phasor approach used in this work appears ideally suited to handle the large amount of data generated by this type of very large sensor (512×512 pixels), even in the case of small number of gates and limited photon budget.