TY - JOUR T1 - An alternative mode of epithelial polarity in the <em>Drosophila</em> midgut JF - bioRxiv DO - 10.1101/307579 SP - 307579 AU - Jia Chen AU - Aram-Christopher Sayadian AU - Nick Lowe AU - Holly E. Lovegrove AU - Daniel St Johnston Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/24/307579.abstract N2 - Apical-basal polarity is essential for the formation and function of epithelial tissues, whereas loss of polarity is a hallmark of tumours. Studies in Drosophila have identified conserved polarity factors that define the apical (Crumbs, Stardust, Par-6, aPKC), junctional (Baz/Par-3) and basolateral (Scribbled, Discs large, Lgl) domains of epithelial cells (1, 2). Because these conserved factors mark equivalent domains in diverse vertebrate and invertebrate epithelial types, it is generally assumed that this system organises polarity in all epithelia. Here we show that this is not the case, as none of these canonical factors are required for the polarisation of the endodermal epithelium of the Drosophila adult midgut. Furthermore, unlike other Drosophila epithelia, the midgut forms occluding junctions above adherens junctions, as in vertebrates, and requires the integrin adhesion complex for polarity (3, 4). Thus, Drosophila contains two types of epithelia that polarise by different mechanisms. Since knock-outs of canonical polarity factors often have little effect on the polarity of vertebrate epithelia, this diversity of polarity mechanisms is likely to be conserved in other animals (5-8). ER -