PT  - JOURNAL ARTICLE
AU  - Simonas Juzenas
AU  - Carl Mårten Lindqvist
AU  - Go Ito
AU  - Yewgenia Dolshanskaya
AU  - Jonas Halfvarson
AU  - Andre Franke
AU  - Georg Hemmrich-Stanisak
TI  - Erythropoietic miR-486-5p and miR-451a depletion from whole blood-derived small RNA sequencing libraries
AID  - 10.1101/789891
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 789891
4099  - http://biorxiv.org/content/early/2019/10/10/789891.short
4100  - http://biorxiv.org/content/early/2019/10/10/789891.full
AB  - Erythroid-specific miR-451a and miR-486-5p are two dominant microRNAs (miRNAs) in human peripheral blood. In small RNA sequencing libraries their overabundance reduces diversity as well as complexity and consequently cause negative effects such as missing detectability and inaccurate quantification of low abundant miRNAs. Here we present a cost-effective and easy to implement hybridization-based method to deplete these two erythropoietic miRNAs from blood-derived RNA samples. By utilization of blocking oligonucleotides, this method provides a highly efficient and specific depletion of miR-486-5p and miR-451a, which leads to a considerable increase of measured expression as well as detectability of low abundant miRNA species. The blocking oligos are compatible with 5’ ligation-dependent small RNA library preparation protocols, including commercially available kits, such as Illumina TruSeq and Perkin Elmer NEXTflex.