PT - JOURNAL ARTICLE AU - Jeziel D. Damasceno AU - João Reis-Cunha AU - Kathryn Crouch AU - Craig Lapsley AU - Luiz R. O. Tosi AU - Daniella Bartholomeu AU - Richard McCulloch TI - Conditional knockout of RAD51-related genes in <em>Leishmania major</em> reveals a critical role for homologous recombination during genome replication AID - 10.1101/800573 DP - 2019 Jan 01 TA - bioRxiv PG - 800573 4099 - http://biorxiv.org/content/early/2019/10/10/800573.short 4100 - http://biorxiv.org/content/early/2019/10/10/800573.full AB - Homologous recombination (HR) has an intimate relationship with genome replication, both during repair of DNA lesions that might prevent DNA synthesis and in tackling stalls to the replication fork. Recent studies led us to ask if HR might have a more central role in replicating the genome of Leishmania, a eukaryotic parasite. Conflicting evidence has emerged regarding whether or not HR genes are essential, and genome-wide mapping has provided evidence for an unorthodox organisation of DNA replication initiation sites, termed origins. To answer this question, we have employed a combined CRISPR/Cas9 and DiCre approach to rapidly generate and assess the effect of conditional ablation of RAD51 and three RAD51-related proteins in Leishmania major. Using this approach, we demonstrate that loss of any of these HR factors is not immediately lethal, but in each case growth slows with time and leads to DNA damage, accumulation of cells with aberrant DNA content, and genome-wide mutation. Despite these similarities, we show that only loss of RAD51 and RAD51-3 impairs DNA synthesis, and that the factors act in distinct ways. Finally, we reveal that loss of RAD51 has a profound effect on DNA replication, causing loss of initiation at the major origins and increased DNA synthesis at subtelomeres. Our work clarifies questions regarding the importance of HR to survival of Leishmania and reveals an unanticipated, central role for RAD51 in the programme of genome replication in a microbial eukaryote.