RT Journal Article
SR Electronic
T1 GAL4/GFP enhancer-trap lines for identification and manipulation of cells and tissues in developing Arabidopsis leaves
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 801357
DO 10.1101/801357
A1 Brindhi Amalraj
A1 Priyanka Govindaraju
A1 Anmol Krishna
A1 Dhruv Lavania
A1 Nguyen Manh Linh
A1 Sree Janani Ravichandran
A1 Enrico Scarpella
YR 2019
UL http://biorxiv.org/content/early/2019/10/10/801357.abstract
AB Understanding developmental processes requires the unambiguous identification of cells and tissues, and the selective manipulation of the properties of those cells and tissues. Both requirements can most efficiently be satisfied through the use of GAL4/GFP enhancer-trap lines. No such lines are available, however, for the study of leaf development in the Columbia-0 reference genotype of Arabidopsis. Here we address this limitation by identifying and characterizing a set of GAL4/GFP enhancer-trap lines in the Columbia-0 background for the specific labeling of cells and tissues during leaf development, and for the targeted expression of genes of interest in those cells and tissues. By using one line in our set to resolve outstanding problems in leaf vein patterning, we show that these lines can be used to address key questions in plant developmental biology.