PT  - JOURNAL ARTICLE
AU  - Rongqun Guo
AU  - Fangxiao Hu
AU  - Qitong Weng
AU  - Cui Lv
AU  - Hongling Wu
AU  - Lijuan Liu
AU  - Zongcheng Li
AU  - Yang Zeng
AU  - Zhijie Bai
AU  - Mengyun Zhang
AU  - Yuting Liu
AU  - Xiaofei Liu
AU  - Chengxiang Xia
AU  - Tongjie Wang
AU  - Peiqing Zhou
AU  - Kaitao Wang
AU  - Yong Dong
AU  - Yuxuan Luo
AU  - Xiangzhong Zhang
AU  - Yuxian Guan
AU  - Yang Geng
AU  - Juan Du
AU  - Yangqiu Li
AU  - Yu Lan
AU  - Jiekai Chen
AU  - Bing Liu
AU  - Jinyong Wang
TI  - Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors
AID  - 10.1101/660977
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 660977
4099  - http://biorxiv.org/content/early/2019/10/12/660977.short
4100  - http://biorxiv.org/content/early/2019/10/12/660977.full
AB  - Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells is a central aim in T cell regenerative medicine. To date, preferentially regenerating T lymphopoiesis in vivo from pluripotent stem cells (PSC) remains a practical challenge. Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial to hematopoietic transition and hematopoietic maturation stages induced in vitro from PSC (iR9-PSC) preferentially generated engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T (iT) cells in primary and secondary immunodeficient recipients. Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSC, unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors. The iT cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβ repertoire. The regenerative T lymphopoiesis rescued the immune-surveillance ability in immunodeficient mice. Furthermore, gene-edited iR9-PSC produced tumor-specific-T cells in vivo that effectively eradicated tumor cells. This study provides insight into universal generation of functional and therapeutic T lymphopoiesis from the unlimited and editable PSC source.