TY - JOUR T1 - Radially-patterned cell behaviours during tube budding from an epithelium JF - bioRxiv DO - 10.1101/308957 SP - 308957 AU - Yara E. Sanchez-Corrales AU - Guy B. Blanchard AU - Katja Röper Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/26/308957.abstract N2 - The budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live imaging and novel morphometric methods we show that in addition to apical constriction, radially oriented directional intercalation of placodal cells plays a major contribution to the early stages of invagination of the salivary gland tube in the Drosophila embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell wedging, and further from the pit cells interleave circumferentially, suggesting apically driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding depends on a radially polarised pattern of apical myosin leading to radially oriented 3D cell behaviours, with a close mechanical interplay between invagination and intercalation. ER -