RT Journal Article
SR Electronic
T1 Identification and comparison of genes differentially regulated by transcription factors and miRNAs
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 803643
DO 10.1101/803643
A1 Amir Asiaee
A1 Zachary B Abrams
A1 Samantha Nakayiza
A1 Deepa Sampath
A1 Kevin R. Coombes
YR 2019
UL http://biorxiv.org/content/early/2019/10/13/803643.abstract
AB Transcription factors and microRNAs (miRNA) both play a critical role in gene regulation and in the development of many diseases such as cancer. Understanding how transcription factors and miRNAs influence gene expression is thus important to understand, but complicated due to the large and interconnected nature of the human genome. To help better understand what genes are being regulated by transcription factors and/or miRNAs we looked at it over 8000 patient samples from 32 different cancer types collected from The Cancer Genome Atlas (TCGA). We started by clustering the transcription factors and miRNAs using Thresher to reduce the number of features. Using both the mRNA and miRNA sequencing data we constructed linear models to calculate the coefficient of determination (R2) for each mRNA based on the Thresher cluster expression. We generated three types of linear models: transcription factor, miRNA and transcription factor plus miRNA. We then determined genes that were highly explained or poorly explained by each of the three models based on the genes R2 value. We performed downstream gene enrichment analysis using ToppGene on the sets of well and poorly explained genes. This identified differences in gene regulation between transcription factors and miRNAs and showed what groups of gene are differentially regulated.