RT Journal Article SR Electronic T1 Experimental increases in glucocorticoids alter function of the neuroendocrine stress axis in wild red squirrels without negatively impacting survival and reproduction JF bioRxiv FD Cold Spring Harbor Laboratory SP 309278 DO 10.1101/309278 A1 Freya van Kesteren A1 Brendan Delehanty A1 Sarah E. Westrick A1 Rupert Palme A1 Rudy Boonstra A1 Jeffrey E. Lane A1 Stan Boutin A1 Andrew G. McAdam A1 Ben Dantzer YR 2018 UL http://biorxiv.org/content/early/2018/04/26/309278.abstract AB Hormones including glucocorticoids (stress hormones) are well known for their effects on animal behavior and life history traits, and this understanding has come through both correlative and manipulative studies. While the latter offers a higher level of control (and ability to assign causality), there are important methodological concerns that are often not considered when manipulating hormones, including glucocorticoids, in wild animals. In this study, we examined how experimental elevations of cortisol concentrations in wild North American red squirrels (TamiasciuŠ³us hudsonicus)affected their hypothalamic-pituitary-adrenal (HPA) axis reactivity, and life history traits including body mass, litter survival, and adult survival. The effects of exogenous cortisol on plasma cortisol concentrations depended on the time between treatment consumption and blood sampling. In the first nine hours after consumption of exogenous cortisol, individuals had significantly higher true baseline plasma cortisol concentrations, but adrenal gland function was impaired. Approximately 24 hours after consumption of exogenous cortisol, individuals had much lower plasma cortisol concentrations than controls, but adrenal function was restored. Corticosteroid binding globulin (CBG) concentrations were also significantly reduced in squirrels treated with cortisol. Despite these profound shifts in the functionality of the neuroendocrine stress axis, fitness proxies including squirrel body mass, offspring survival, and adult survival were unaffected by experimental increases in cortisol concentrations. Our results highlight that even short-term experimental increases in glucocorticoids can affect adrenal gland functioning and CBG concentrations, but may have no side-effects on proxies of fitness.