TY - JOUR T1 - High-throughput chromatin accessibility profiling at single-cell resolution JF - bioRxiv DO - 10.1101/310284 SP - 310284 AU - Anja Mezger AU - Sandy Klemm AU - Ishminder Mann AU - Kara Brower AU - Alain Mir AU - Magnolia Bostick AU - Andrew Farmer AU - Polly Fordyce AU - Sten Linnarsson AU - William Greenleaf Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/28/310284.abstract N2 - We have developed a high-throughput single-cell ATAC-seq (assay for transposition of accessible chromatin) method to measure physical access to DNA in whole cells. Our approach integrates fluorescence imaging and addressable reagent deposition across a massively parallel (5184) nano-well array, yielding a nearly 20-fold improvement in throughput (up to ~1800 cells/chip, 4-5 hour on-chip processing time) and cost (~98ยข per cell) compared to prior microfluidic implementations. We applied this method to measure regulatory variation in Peripheral Blood Mononuclear Cells (PBMCs) and show robust, de-novo clustering of single cells by hematopoietic cell type. ER -