PT  - JOURNAL ARTICLE
AU  - Mezger, Anja
AU  - Klemm, Sandy
AU  - Mann, Ishminder
AU  - Brower, Kara
AU  - Mir, Alain
AU  - Bostick, Magnolia
AU  - Farmer, Andrew
AU  - Fordyce, Polly
AU  - Linnarsson, Sten
AU  - Greenleaf, William
TI  - High-throughput chromatin accessibility profiling at single-cell resolution
AID  - 10.1101/310284
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 310284
4099  - http://biorxiv.org/content/early/2018/04/28/310284.short
4100  - http://biorxiv.org/content/early/2018/04/28/310284.full
AB  - We have developed a high-throughput single-cell ATAC-seq (assay for transposition of accessible chromatin) method to measure physical access to DNA in whole cells. Our approach integrates fluorescence imaging and addressable reagent deposition across a massively parallel (5184) nano-well array, yielding a nearly 20-fold improvement in throughput (up to ~1800 cells/chip, 4-5 hour on-chip processing time) and cost (~98¢ per cell) compared to prior microfluidic implementations. We applied this method to measure regulatory variation in Peripheral Blood Mononuclear Cells (PBMCs) and show robust, de-novo clustering of single cells by hematopoietic cell type.