RT Journal Article
SR Electronic
T1 Measuring chromosome conformation by fluorescence microscopy
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 798512
DO 10.1101/798512
A1 Brian C. Ross
A1 Fabio Anaclerio
A1 Nicola Lorusso
A1 Mario Ventura
A1 Jim Costello
YR 2019
UL http://biorxiv.org/content/early/2019/10/16/798512.abstract
AB Measurement of in-vivo chromosome conformations (structures) in single cells is a major technological goal of structural biology. If one could identify many genetic loci in a microscope image despite the limited palette of fluorescent colors used to label them, then the conformation could be solved at some resolution by ‘connecting the dots’. Computational tools for making this reconstruction are expected to produce near-perfect reconstructions when the number of fluorescent colors is high enough, irrespective of the number of loci assayed. Here we report the first experimental test of the performance of these reconstruction algorithms and check their ability to reconstruct experimentally-measured conformations. We also demonstrate the experimental metrics needed to assess reconstruction quality. Our results indicate that current sequential FISH experiments may be close to the point where the reconstructions are nearly flawless at some distance scales.