RT Journal Article
SR Electronic
T1 Discovery of a new path for red blood cell generation in the mouse embryo
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 309054
DO 10.1101/309054
A1 Irina Pinheiro
A1 Özge Vargel Bölükbaşi
A1 Kerstin Ganter
A1 Laura A. Sabou
A1 Vick Key Tew
A1 Giulia Bolasco
A1 Maya Shvartsman
A1 Polina V. Pavlovich
A1 Andreas Buness
A1 Christina Nikolakopoulou
A1 Isabelle Bergiers
A1 Valerie Kouskoff
A1 Georges Lacaud
A1 Christophe Lancrin
YR 2018
UL http://biorxiv.org/content/early/2018/04/28/309054.abstract
AB Erythropoiesis occurs through several waves during embryonic development. Although the source of the primitive wave is well characterized, the origin of erythrocytes later in embryogenesis is less clear due to overlaps between the different erythroid waves. Using the miR144/451-GFP mouse model to track cells expressing the erythroid microRNAs miR144/451, we identified cells co-expressing VE-Cadherin and GFP in the yolk sac between E9.5 and E12. This suggested the existence of hemogenic endothelial cells committed to erythropoiesis (Ery-HEC). We showed that these cells were capable of generating erythrocytes ex vivo and we demonstrated that the formation of Ery-HEC was independent of the Runx1 gene expression. Using transcriptome analysis, we demonstrated that these cells coexpressed endothelial and erythroid genes such as Hbb-bh1 and Gata1 but we were surprised to detect the primitive erythroid genes Aqp3 and Aqp8 suggesting the formation of primitive erythrocytes at a much later time point than initially thought. Finally, we showed that enforced expression of Gata1 in endothelial cells was enough to initiate the erythroid transcriptional program.