RT Journal Article
SR Electronic
T1 Transcriptional Profiling of Primate Central Nucleus of the Amygdala Neurons to Understand the Molecular Underpinnings of Early Life Anxious Temperament
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 808279
DO 10.1101/808279
A1 Rothem Kovner
A1 Tade Souaiaia
A1 Andrew S. Fox
A1 Delores A. French
A1 Cooper. E. Goss
A1 Patrick H. Roseboom
A1 Jonathan A. Oler
A1 Marissa K. Riedel
A1 Eva M. Fekete
A1 Julie L. Fudge
A1 James A. Knowles
A1 Ned H. Kalin
YR 2019
UL http://biorxiv.org/content/early/2019/10/17/808279.abstract
AB Children exhibiting extreme anxious temperament (AT) are at an increased risk to develop anxiety and depression. Work in young rhesus monkeys mechanistically links the central nucleus of the amygdala (Ce) to AT. Here, we used laser capture microscopy and RNA sequencing in 47 young rhesus monkeys to investigate AT‘s molecular underpinnings by focusing on lateral Ce (CeL) neurons. We found 528 AT-related transcripts, including protein kinase C type-delta (PKCδ), a CeL microcircuit cell marker implicated in rodent threat processing. We characterized PKCδ neurons in the rhesus CeL, compared their distribution to the mouse, and demonstrated that a subset of these neurons project to the laterodorsal bed nucleus of the stria terminalis (BSTLd). These findings present evidence in the primate of a CeL to BSTLd circuit that maybe relevant to understanding human anxiety and points to specific molecules within this circuit that could serve as potential treatment targets for anxiety disorders.