PT - JOURNAL ARTICLE AU - Yansheng Liu AU - Yang Mi AU - Torsten Mueller AU - Saskia Kreibich AU - Evan G. Williams AU - Audrey Van Drogen AU - Christelle Borel AU - Pierre-Luc Germain AU - Max Frank AU - Isabell Bludau AU - Martin Mehnert AU - Michael Seifert AU - Mario Emmenlauer AU - Isabel Sorg AU - Fedor Bezrukov AU - Frederique Sloan Bena AU - Hu Zhou AU - Christoph Dehio AU - Giuseppe Testa AU - Julio Saez-Rodriguez AU - Stylianos E. Antonarakis AU - Wolf-Dietrich Hardt AU - Ruedi Aebersold TI - Genomic, Proteomic and Phenotypic Heterogeneity in HeLa Cells across Laboratories: Implications for Reproducibility of Research Results AID - 10.1101/307421 DP - 2018 Jan 01 TA - bioRxiv PG - 307421 4099 - http://biorxiv.org/content/early/2018/04/30/307421.short 4100 - http://biorxiv.org/content/early/2018/04/30/307421.full AB - The independent reproduction of research results is a cornerstone of experimental research, yet it is beset by numerous challenges, including the quality and veracity of reagents and materials. Much of life science research depends on life materials, including human tissue culture cells. In this study we aimed at determining the degree of variability in the molecular makeup and the ensuing phenotypic consequences in commonly used human tissue culture cells. We collected 14 stock HeLa aliquots from 13 different laboratories across the globe, cultured them in uniform conditions and profiled the genome-wide copy numbers, mRNAs, proteins and protein turnover rates via genomic techniques and SWATH mass spectrometry, respectively. We also phenotyped each cell line with respect to the ability of transfected Let7 mimics to modulate Salmonella infection.We discovered significant heterogeneity between HeLa variants, especially between lines of the CCL2 and Kyoto variety. We also observed progressive divergence within a specific cell line over 50 successive passages. From the aggregate multi-omic datasets we quantified the response of the cells to genomic variability across the transcriptome and proteome. We discovered organelle-specific proteome remodeling and buffering of protein abundance by protein complex stoichiometry, mediated by the adaptation of protein turnover rates. By associating quantitative proteotype and phenotype measurements we identified protein patterns that explained the varying response of the different cell lines to Salmonella infection.Altogether the results indicate a striking degree of genomic variability, the rapid evolution of genomic variability in culture and its complex translation into distinctive expressed molecular and phenotypic patterns. The results have broad implications for the interpretation and reproducibility of research results obtained from HeLa cells and provide important basis for a general discussion of the value and requirements for communicating research results obtained from human tissue culture cells.