RT Journal Article
SR Electronic
T1 Plants with self-sustained luminescence
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 809376
DO 10.1101/809376
A1 Tatiana Mitiouchkina
A1 Alexander S. Mishin
A1 Louisa Gonzalez Somermeyer
A1 Nadezhda M. Markina
A1 Tatiana V. Chepurnyh
A1 Elena B. Guglya
A1 Tatiana A. Karataeva
A1 Kseniia A. Palkina
A1 Ekaterina S. Shakhova
A1 Liliia I. Fakhranurova
A1 Sofia V. Chekova
A1 Aleksandra S. Tsarkova
A1 Yaroslav V. Golubev
A1 Vadim V. Negrebetsky
A1 Sergey A. Dolgushin
A1 Pavel V. Shalaev
A1 Olesya A. Melnik
A1 Victoria O. Shipunova
A1 Sergey M. Deyev
A1 Andrey I. Bubyrev
A1 Alexander S. Pushin
A1 Vladimir V. Choob
A1 Sergey V. Dolgov
A1 Fyodor A. Kondrashov
A1 Ilia V. Yampolsky
A1 Karen S. Sarkisyan
YR 2019
UL http://biorxiv.org/content/early/2019/10/18/809376.abstract
AB In contrast to fluorescent proteins, light emission from luciferase reporters requires exogenous addition of a luciferin substrate. Bacterial bioluminescence has been the single exception, where an operon of five genes is sufficient to produce light autonomously. Although commonly used in prokaryotic hosts, toxicity of the aldehyde substrate has limited its use in eukaryotes1. Here we demonstrate autonomous luminescence in a multicellular eukaryotic organism by incorporating a recently discovered fungal bioluminescent system2 into tobacco plants. We monitored these light-emitting plants from germination to flowering, observing temporal and spatial patterns of luminescence across time scales from seconds to months. The dynamic patterns of luminescence reflected progression through developmental stages, circadian oscillations, transport, and response to injuries. As with other fluorescent and luminescent reporters, we anticipate that this system will be further engineered for varied purposes, especially where exogenous addition of substrate is undesirable.