PT  - JOURNAL ARTICLE
AU  - Kakeru Yokoi
AU  - Takuya Tsubota
AU  - Jianqiang Sun
AU  - Akiya Jouraku
AU  - Hideki Sezutsu
AU  - Hidemasa Bono
TI  - Reference transcriptome data in silkworm <em>Bombyx mori</em>
AID  - 10.1101/805978
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 805978
4099  - http://biorxiv.org/content/early/2019/10/18/805978.short
4100  - http://biorxiv.org/content/early/2019/10/18/805978.full
AB  - The silkworm Bombyx mori has long been used in the silk industry and utilized in studies of physiology, genetics, molecular biology, and pathology. We recently reported high quality reference genome data for B. mori. In the present study, we constructed a reference transcriptome data set using the reference genome data and RNA-seq data of 10 tissues from P50T strain larvae. Reference transcriptome data contained 51,926 transcripts, with 39,619 having one or more coding sequence region. The abundance of each transcript in the 10 tissues as well as 5 tissues from other strain larvae was estimated, and hierarchical clustering was performed. The results obtained showed that data on abundance were highly reproducible and there here is a little difference of transcriptome abundance between the two strain larvae. New isoforms of silk-related genes were searched for in the reference transcriptomes, and the longest isoform of sericin-1 possessing a long exon was identified. We also extracted transcripts that were strongly expressed in one or more parts of the silk glands. An enrichment analysis performed using the functional annotation data of the transcripts provided novel insights into the functions of the silk gland parts. Therefore, the reference transcriptome data set obtained has extended B. mori genomic and transcriptomic insights and may contribute to advances in entomologic and vertebrate research, including that on humans.