PT  - JOURNAL ARTICLE
AU  - Fioranna Renda
AU  - Valentin Magidson
AU  - Irina Tikhonenko
AU  - Christopher Miles
AU  - Alex Mogilner
AU  - Alexey Khodjakov
TI  - kSHREC ‘Delta’ reflects the shape of kinetochore rather than intrakinetochore tension
AID  - 10.1101/811075
DP  - 2019 Jan 01
TA  - bioRxiv
PG  - 811075
4099  - http://biorxiv.org/content/early/2019/10/18/811075.short
4100  - http://biorxiv.org/content/early/2019/10/18/811075.full
AB  - Distance between fluorescent spots formed by various kinetochore proteins (‘Delta’) is proposed to reflect the level of intrakinetochore tension (IKT). However, larger-scale changes in the kinetochore architecture may also affect Delta. To test this possibility, we measure Delta in long kinetochores of Indian muntjac (IM) whose shape, size, and orientation are discernable in conventional light microscopy. We find that architecture of IM kinetochores and the value of Delta change minimally when microtubule-mediated forces are suppressed by Taxol. In contrast, large decreases of Delta observed in Taxol-treated human cells coincide with prominent changes in length and shape of the kinetochore. We also find that inner and outer kinetochore proteins intermix within a common spatial compartment instead of forming separate thin layers. These observations, supported by computational modelling, suggest that changes in Delta reflect changes in the kinetochore shape rather than the level of IKT.