RT Journal Article
SR Electronic
T1 kSHREC ‘Delta’ reflects the shape of kinetochore rather than intrakinetochore tension
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 811075
DO 10.1101/811075
A1 Fioranna Renda
A1 Valentin Magidson
A1 Irina Tikhonenko
A1 Christopher Miles
A1 Alex Mogilner
A1 Alexey Khodjakov
YR 2019
UL http://biorxiv.org/content/early/2019/10/18/811075.abstract
AB Distance between fluorescent spots formed by various kinetochore proteins (‘Delta’) is proposed to reflect the level of intrakinetochore tension (IKT). However, larger-scale changes in the kinetochore architecture may also affect Delta. To test this possibility, we measure Delta in long kinetochores of Indian muntjac (IM) whose shape, size, and orientation are discernable in conventional light microscopy. We find that architecture of IM kinetochores and the value of Delta change minimally when microtubule-mediated forces are suppressed by Taxol. In contrast, large decreases of Delta observed in Taxol-treated human cells coincide with prominent changes in length and shape of the kinetochore. We also find that inner and outer kinetochore proteins intermix within a common spatial compartment instead of forming separate thin layers. These observations, supported by computational modelling, suggest that changes in Delta reflect changes in the kinetochore shape rather than the level of IKT.