TY - JOUR T1 - Changes in the gut microbiota and fermentation products associated with enhanced longevity in acarbose-treated mice JF - bioRxiv DO - 10.1101/311456 SP - 311456 AU - Byron J Smith AU - Richard A Miller AU - Aaron C Ericsson AU - David C Harrison AU - Randy Strong AU - Thomas M Schmidt Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/04/30/311456.abstract N2 - Background Treatment with the α-glucosidase inhibitor acarbose increases median lifespan by approximately 20% in male mice and 5% in females. This longevity extension differs from dietary restriction based on a number of features, including the relatively small effects on weight and the sex-specificity of the lifespan effect. By inhibiting host digestion, acarbose increases the flux of starch to the lower digestive system, resulting in changes to the gut microbiota and their fermentation products. Given the documented health benefits of short-chain fatty acids (SCFAs), the dominant products of starch fermentation by gut bacteria, this secondary effect of acarbose could contribute to increased longevity in mice. To explore this hypothesis, we compared the fecal microbiome of mice treated with acarbose to control mice at three independent study sites.Results Microbial communities and the concentrations of SCFAs in the feces of mice treated with acarbose were notably different from those of control mice. At all three study sites, the bloom of a single bacterial taxon was the most obvious response to acarbose treatment. The blooming populations were classified to the largely uncultured Bacteroidales family Muribaculaceae and were the same taxonomic unit at two of the three sites. Total SCFA concentrations in feces were increased in treated mice, with increased butyrate and propionate in particular. Across all samples, Muribaculaceae abundance was strongly correlated with propionate and community composition was an important predictor of SCFA concentrations. Cox proportional hazards regression showed that the fecal concentrations of acetate, butyrate, and propionate were, together, predictive of mouse longevity even while controlling for sex, site, and acarbose.Conclusion We have demonstrated a correlation between fecal SCFAs and lifespan in mice, suggesting a role of the gut microbiota in the longevity-enhancing properties of acarbose. Treatment modulated the taxonomic composition and fermentation products of the gut microbiome, while the site-dependence of the microbiota illustrates the challenges facing reproducibility and interpretation in microbiome studies. These results motivate future studies exploring manipulation of the gut microbial community and its fermentation products for increased longevity, and to test a causal role of SCFAs in the observed effects of acarbose.ACA: AcarboseSCFA: Short-chain fatty acidITP: Interventions Testing ProgramTJL: The Jackson LaboratoryUM: The University of MichiganUT: The University of Texas Health Science Center at San AntonioOTU: Operational taxonomic unitHPLC: High-performance liquid chromatographyLASSO: Least absolute shrinkage and selection operationS. alaskensis: Sphingopyxis alaskensisFDR: False-discovery ratePBS: Phosphate-buffered saline ER -