PT  - JOURNAL ARTICLE
AU  - Pham, Tri Thanh
AU  - Monnard, Arnaud
AU  - Helenius, Jonne
AU  - Lund, Erik
AU  - Lee, Nicole
AU  - Müller, Daniel J.
AU  - Cabernard, Clemens
TI  - Spatiotemporally controlled Myosin relocalization and internal pressure cause biased cortical extension to generate sibling cell size asymmetry
AID  - 10.1101/311852
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 311852
4099  - http://biorxiv.org/content/early/2018/05/01/311852.short
4100  - http://biorxiv.org/content/early/2018/05/01/311852.full
AB  - Metazoan cells can generate unequal sized sibling cells during cell division. This form of asymmetric cell division depends on spindle geometry and Myosin distribution but the underlying mechanics are unclear. Here, we use atomic force microscopy and live cell imaging to elucidate the biophysical forces involved in the establishment of physical asymmetry in Drosophila neural stem cells. We show that the force driving initial apical membrane expansion is provided by hydrostatic pressure, peaking shortly after anaphase onset, and enabled by a relieve of actomyosin contractile tension on the apical cell cortex. The subsequent increase in contractile forces at the cleavage furrow, combined with the relocalization of basally located Myosin results in basal membrane extension and sustained apical expansion. We propose that spatiotemporally controlled actomyosin contractile tension and hydrostatic pressure enables stereotypic biased membrane expansion to generate sibling cell size asymmetry.