TY - JOUR T1 - Protein Phosphatase 1 activity controls a balance between collective and single cell modes of migration JF - bioRxiv DO - 10.1101/811562 SP - 811562 AU - Yujun Chen AU - Nirupama Kotian AU - George Aranjuez AU - Lin Chen AU - C. Luke Messer AU - Ashley Burtscher AU - Ketki Sawant AU - Damien Ramel AU - Xiaobo Wang AU - Jocelyn A. McDonald Y1 - 2019/01/01 UR - http://biorxiv.org/content/early/2019/10/20/811562.abstract N2 - Collective cell migration is central to many developmental and pathological processes. However, the mechanisms that keep cell collectives together and coordinate movement of multiple cells are poorly understood. Using the Drosophila border cell migration model, we find that Protein phosphatase 1 (Pp1) activity controls collective cell cohesion and migration. Inhibition of Pp1 causes border cells to round up, dissociate, and move as single cells with altered motility. We present evidence that Pp1 promotes proper levels of cadherin-catenin complex proteins at cell-cell junctions within the cluster to keep border cells together. Pp1 further restricts actomyosin contractility to the cluster periphery rather than at internal cell-cell contacts. We show that the myosin phosphatase Pp1 complex, which inhibits non-muscle myosin-II (Myo-II) activity, coordinates border cell shape and cluster cohesion. Given the high conservation of Pp1 complexes, this study identifies Pp1 as a major regulator of collective versus single cell migration. ER -